ea0016me4 | (1) | ECE2008
Friedl Peter
Multiphoton microscopy has defined standards for 3D fluorescence and higher harmonic generation analysis of cells and tissue structures in vitro and in vivo. Compared to single-photon excited confocal microscopy, two-photon microscopy utilizes near-infrared (NIR) excitation generating twice to multi-fold enhanced tissue penetration, reduced light scattering and minimized phototoxicity and photobleaching at out-of-focus regions, yet preserves submicron spatial res...